PrimerDriver is a user-friendly bioinformatics platform specifically designed to generate primers for site-directed mutagenesis experiments into workflows with an easy-to-use command-line interface. "Traversing" PrimerDriver lanes, users can generate possible mutagenic primers upon input of a DNA sequence (DNA), design primers for an array of species codon expression systems through direct mutation of the amino acid (PRO), characterize and report user-designed primers (CHAR).